Fig. 5.
Small peritoneal macrophages express a proangiogenic gene profile and directly promote tumor cell proliferation. (A) F4/80hi (LPM) and F4/80lo (SPM) macrophages from the peritoneal exudates of C57BL/6 wild type were FACS sorted after 6 wk of ID8 tumor development and analyzed by RT-qPCR for tnfa, tgfb, vegfa, cxcl1, cxcl8, il10, il1b, il6, il17ra, mif, tie2, and cd163 expression, normalized to the housekeeping gene hprt. Bars represent SD; *P < 0.05, **P < 0.01, ***P < 0.005. (B and C) ID8 tumor cell numbers (at 24, 48, and 96 h) and BrdU incorporation (at 48 h) upon noncontact coculture (at 1:1 ratio) with LPMs or SPMs, sorted from ID8 tumor-bearing mice (at week 6). Data are representative of two to three independent experiments; **P < 0.01.