Skip to main content
. 2014 Aug 11;111(34):12556–12561. doi: 10.1073/pnas.1319488111

Fig. 2.

Fig. 2.

(A) Dose–response of KJ-Pyr-9 versus oncogenic transformation induced by MYC, N-MYC, v-Src, PIK3CA-H1047R, and v-Jun. Data from a representative experiment conducted with cells derived from a single chicken embryo. In these transformation assays, the MYC expression vector produced the larger isoform of MYC, initiated by the first initiation codon that had been mutated from CTG to ATG. Identical results were obtained by expressing MYC from the wild-type mRNA sequence and from an mRNA sequence in which the first initiation codon CTG is mutated to a nonfunctional CAG, thus forcing expression of only the smaller isoform of MYC. EOT, efficiency of transformation. (B) CEF were infected at a multiplicity of infection of 10 with the RCAS retroviral vector expressing ATG-MYC. Cells were transferred on days 3 and 5 postinfection and treated or not treated with KJ-Pyr-9, and cleaved caspase 3 was determined by Western blot on day 9.