Uptake and gene delivery studies of mannosylated D9 hybrid vectors. (A) Uptake of bacterial and hybrid vectors in the presence of increasing concentrations of mannose. (B) Gene delivery of hybrid vectors at three MOIs. Positive controls included FuGENE 6 (Promega), FuGENE HD (Promega), ViaFECT (Promega), OmniFect (TransOMIC Technologies), Xfect (Clontech Laboratories), JET-PEI (Polyplus transfection), and GeneJuice (EMD Millipore). *Statistical significance (95% confidence) compared with associated YWT7-hly/pRSET-emerald green GFP (EmGFP) and YWT7-hly/pRSET-EmGFP:D9 datasets (A) or S1 and synthetic controls (B). #Statistical significance (95% confidence) indicates reduced bacterial uptake at each mannose concentration.