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VipD localization to endosomes requires a functional Rab5 binding interface. Transiently transfected COS-1 cells producing Rab5a(Q79L) and the indicated mCherry-tagged VipD variants were analyzed by fluorescence microscopy to determine protein localization. The merged images (bottom row) show Rab5a(Q79L) in green and VipD variants in red. (Insets) Magnified view of endosomes marked by an arrowhead. Control, mCherry. (Scale bar, 2 μm.)
