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. 2014 Aug 13;111(34):E3544–E3552. doi: 10.1073/pnas.1413201111

Fig. 2.

Fig. 2.

Calcineurin activation is central to α-syn toxicity in yeast. (A) Control and HiTox strains lacking calcineurin (cnb1Δ) were spotted onto plates containing uninducing media (SD −His,Trp–α-syn selective, Lower) and replica plated in threefold serial dilutions on α-syn–inducing plates containing selective media SGal (Upper). (B) Control and HiTox yeast strains were spotted onto plates containing uninducing media (SD −Ura, Leu; cna1- and cnb1 selective; Lower) and replica plated in threefold serial dilutions onto α-syn–inducing plates containing selective media and SGal (Upper). (C) Same assay as in B except control and HiTox strains was plated onto plates containing uninducing media (SD −Leu; rcn1-, or rcn2 selective, respectively). (D) Growth (described as percentage over control) of HiTox cells grown for 48 h over a range of FK506 concentrations. See also Fig. S4D.