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. 2014 Aug 11;111(34):E3571–E3580. doi: 10.1073/pnas.1403851111

Fig. 6.

Fig. 6.

DOG1 influences the expression of GA biosynthesis and CAP-weakening genes in a temperature-dependent manner. Temperature-dependent gene-expression profiles of candidate GA biosynthesis and endosperm CAP-weakening genes during the germination of L. sativum E17 seeds overexpressing AtDOG1 compared with WT seeds. qRT-PCR analysis of transcript abundance of (A) GA biosynthesis genes (KO, KAO1, GA20ox, GA3ox1) and (B) candidate cell-wall remodeling genes (EXPA2, EXPA9, EXLA1, XTH19) at 18 °C and 24 °C in WT (Left) and E17 (Right) seeds without (−TR) or with (+TR) testa rupture were sampled at the indicated time points, which relate to the TR kinetics indicated by the gray curves. n = 3; data are shown as mean ± SEM. Results for other seed batches and lines are shown in SI Appendix, Fig. S6. Note that temperature affects transcript abundances and germination differently in WT and E17 seeds, but the overexpressed AtDOG1 transcript and protein amounts themselves were not temperature-regulated in E17 seeds during germination (SI Appendix, Fig. S7).