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. 2014 Aug 12;111(34):12474–12479. doi: 10.1073/pnas.1406240111

Fig. 5.

Fig. 5.

The KLRG1+ iNKT cell recall response. (A) Mice were immunized with or without DC/Gal (100) as a first immunization. Two months later, the mice were administered unloaded DC or α-GalCer–loaded DC (DC/Gal; 10 ng/mL), DC/Gal (100 ng/mL), iGB3 (10 μg/mL)-loaded DC (DC/iGB3), or GSL (10 μg/mL)-loaded DC (DC/GSL) as a second immunization. The number of KLRG1+ iNKT cells in the lung was measured 7 d after the second immunization by flow cytometry after gating on CD19−CD1d-dimer/Gal+ cells. (B) As in A, but immunized mice were analyzed for iNKT cell TCR Vβ using TCR Vβ-FITC. Analysis gates were set on CD19−CD1d-dimer/Gal+KLRG1− cells in the nonimmunized group and CD19−CD1d-dimer/Gal+KLRG1+ cells in the immunized groups. Data are representative of two separate experiments (mean ± SEM); n = 4–6 per group. *P < 0.05 DC/Gal (10) versus DC/Gal-DC/Gal (10) and DC/Gal (100) versus DC/Gal-DC/Gal (100).