Figure 2.

Bioluminescence in control and drug treated P. falciparum gametocytes: (a) d-Luciferin and Britelite performances in drug treatment assay. Bioluminescence from early stage gametocytes expressing CBG99 (left) and CBR (right) measured with d-luciferin (●) and Britelite (▲) at t = 0, t = 24 h, and t = 48 h. (b) Same experiment on stage IV gametocytes. Results are expressed as drug/DMSO ratio. Statistics are performed with the GraphPad Prism software. (c) Comparison of d-luciferin and Britelite performances in dose–response gametocytocidal assays. Early (left) and late (right) stage gametocytes expressing the CBG99 luciferase were treated for 72 h with increasing doses of epoxomicin (0–90 nM) before luciferase activity was measured with 0.5 mM d-luciferin and Britelite. IC₅₀ values were calculated using the GraphPad Prism software.