Figure 4.

Dual-color gametocyte assay validation. (a) Dual-luciferase assay with early and mature gametocytes, respectively, expressing the CBG99 (green bar) and the CBR luciferase (red bar). BL intensities were acquired with a Varioskan luminometer using F545 and F615 optical filters. Raw BL measurements were spectrally unmixed with the Chroma-Luc calculator and normalized with respect to DMSO control. (b) Reciprocal dual-color assay with mature and early stage gametocytes expressing the CBG99 and the CBR luciferase, respectively. (c) Comparison of Britelite and d-luciferin performance in an independent dual-color assay using CBG99 mature stage and CBR early stage gametocytes.