PEI-CNT
fiber microelectrodes maintain antifouling properties in
brain slices. (A,B) Serotonin (25 μM) was exogenously applied
near a PEI-CNT fiber electrode in a brain slices every 2 min for 10
min. (A) The black trace shows the initial serotonin CV, and the dashed
red trace shows the fifth serotonin CV. The CVs are similar. (B) Serotonin
current does not significantly change with ejection number (repeated
measures one-way ANOVA, p = 0.21, n = 6, error bars SEM). (C,D) Exogenous serotonin was applied, the
slice bathed in 10 μM 5-HIAA and serotonin applied again after
30 min, and then the 5-HIAA was washed out and exogenous serotonin
applied again. (C) Serotonin CVs before (black trace), after 30 min
perfusion of 5-HIAA (red trace), and after 5-HIAA washout (blue trace)
are similar. (D) The peak serotonin oxidative current did not significantly
change in the presence of 5-HIAA (repeated measures one-way ANOVA, p = 0.46, n = 6, error bars SEM).