Figure 3.

Compound K enhances autophagy by inhibiting the activity of mTOR. (A) Primary astrocytes were treated with different concentrations of compound K (50, 20, 10, 1 or 0 μM) for 24 h. The total proteins were collected and the phosphorylation levels of P70S6K and mTOR were detected by western blot analysis. (B) Primary astrocytes were treated with different concentrations of compound K (50, 20, 10, 1 or 0 μM) for 24 h. The total proteins were collected and the phosphorylation of ULK1 and the levels of P62 were detected using western blot analysis. mTOR, mammalian target of rapamycin.