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. 2014 Jul 22;45(4):1412–1420. doi: 10.3892/ijo.2014.2557

Figure 2.

Figure 2

Expression of GFP by each transfectant. (A) The assessment of EGFP expression in pIRES2-EGFP vector-transfected fibroblasts. Upper images show the GFP-positive expression of fibroblasts by fluorescence microscopy. Lower images show the positive expression of GFP by flow cytometry. (B) Upper images show the negative expression of GFP on fibroblasts by fluorescence microscopy. Lower images show the negative expression of GFP by flow cytometry. In normal cell lines, TERT-EGFP-transfected fibroblast clones did not express EGFP. (C) Upper, optical microscopy; lower, fluorescence microscopy. In the cancer cell lines, the GFP expression level of TERT-EGFP-transfected colon 26 cells was high. (D) Mean fluorescence intensity of GFP on various human cancer cell lines transfected with pIRES-EGFP vector and hTERT-EGFP vector was determined by flow cytometry.