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Expression of IGFs and IGF-R by the leukemic cells. IGF-I, -II, -IR and -IIR mRNA expression was studied by RT-PCR. β2-microglobulin was used as control (A). The proteins of IGF family were analyzed by immunocytochemistry. As negative controls, the cells were incubated only with the secondary antibodies (B). The amount of IGF-I pg/ml in the supernatants of the sensitive and resistant leukemic cells was analyzed by ELISA (C).
