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FOXO3 is essential for auranofin-mediated apoptosis in SKOV3 cells. SKOV3 cells were transfected with control-siRNA or FOXO3-siRNA for 24 h as described in Materials and methods. Subsequently, the transfected cells were treated with auranofin (100 nM) or control (DMSO) for 48 h and total lysates of cells were analyzed by western blot analysis with specific antibodies against apoptosis-related proteins as indicated. β-actin represents the loading controls.
