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. Author manuscript; available in PMC: 2014 Sep 2.
Published in final edited form as: J Invest Dermatol. 2010 Dec 2;131(4):848–856. doi: 10.1038/jid.2010.346

Figure 1. Induction of RDEB skin cells into RDEB iPS cells.

Figure 1

A, D, G, J, M: Live human iPS cultures stained with TRA-1-60 antibody four weeks after transduction. B, E, H, K, N: Phase contrast image of the same iPS colonies. C, F, I, L, O: Alkaline phophatase stain. P: Quantitative RT-PCR analysis of OCT4, SOX2, NANOG, KLF4, c-MYC, LIN28, and DNMT3b expression levels in RDEB KC iPS cells from P2 and P3 relative to expression levels in parental RDEB KCs from P2 and P3. Q: Quantitative RT-PCR analysis of the same genes in WT KC- iPS cells relative to expression levels in parental WT KCs. All values were normalized against endogenous GAPDH expression. FB, fibroblasts; KC, keratinocytes; WT, wild type; PC, phase contrast; AP, alkaline phosphatase; iPS, induced pluripotent stem cells.