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. 2014 Apr 22;2(3):e00042. doi: 10.1002/prp2.42

Figure 7.

Figure 7

Cardiovascular parameters in 5/6 NX rats on high-phosphate diet. NX rats were treated with 5% vehicle (unprocessed polymer), 5% sevelamer-carbonate, or 5% VS-501 in food for 4 weeks as described above. (A) E/A ratio: Cardiac function was determined at predosing (pre) and Week 4 after dosing (W4) as described in Methods. Statistical comparisons between two groups were performed by unpaired t-test with 95% confidence intervals of difference. #P < 0.05, ##P < 0.01 versus Sham-predosing. *P < 0.05 versus NX-predosing. (B) Calcification staining: Aorta samples were randomly selected from each 5/6 NX group with n = 16 (four aorta samples per rat, four rats per group) and stained by the von Kossa method. The staining was scored separately by two investigators in a blinded manner and the scores were averaged for each sample. Two representative samples indicating 0% and 70% positive staining of the aortic cross-sectional area are shown. (C) Quantification of calcification: The percentages of positive staining in each treatment group were calculated and expressed as mean ± SE. Statistical comparisons between two groups were performed by unpaired t-test with 95% confidence intervals of difference.*P < 0.05 versus Sham.