Figure 8.

Intestinal type II sodium-dependent phosphate cotransporter (NPT2b) gene expression. NX rats were treated with 5% vehicle (unprocessed polymer), 5% sevelamer carbonate, or 5% VS-501 in food for 4 weeks as described above. RNA samples were prepared from small intestines using the standard RNA isolation procedure. The real-time RT-PCR was performed as described in Methods. NPT2b values were normalized with GAPDH. Statistical comparisons between two groups were performed by unpaired t-test with 95% confidence intervals of difference. Mean ± SE was calculated for each group.