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. 1975 Jun;11(6):1163–1169. doi: 10.1128/iai.11.6.1163-1169.1975

In Vitro Lymphocyte Response to Purified Protein Derivative, BCG, and Mycobacterium leprae in a Population Not Exposed to Leprosy

Otto Closs 1
PMCID: PMC415194  PMID: 1095479

Abstract

Lymphocytes from 14 BCG-vaccinated donors, seven tuberculin positive and seven tuberculin negative by skin testing, were stimulated in vitro with four mycobacterial antigens, purified protein derivative (PPD), PPD/BCG, whole BCG bacilli, and whole Mycobacterium leprae and also with Candida antigen and phytohemagglutinin. The response was measured by incorporation of 3H-labeled thymidine. The response to PPD, PPD/BCG, and BCG was found to correlate with the result of skin testing with turberculin. The turberculin-positive group also responded more strongly to M. leprae, whereas the two groups did not differ significantly in their response to Candida antigen or phytohemagglutinin. These findings indicate a certain degree of cross-reactivity between BCG and M. leprae. The use of the lymphocyte transformation test to measure antigenic cross-reactivity is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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