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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: J Pathol. 2014 Jun 9;233(4):380–391. doi: 10.1002/path.4368

Figure 5.

Figure 5

Interrupted Tamoxifen injection induced apoptosis and senescence in the Mdm2FM/−;CreER mice. A, TUNEL staining and ki67 immunohistochemical staining of paraffin-embedded sections of tissues. Caspase 3 staining was alternatively used to detect apoptosis in hippocampus due to extensive non-specific TUNEL staining in the control Mdm2FM/− mouse brain. B, Real-time reverse transcription PCR analysis of the mRNAs of a variety of senescence markers in different tissues. Ctrl or C: the control Mdm2FM/− mice. FM/−;CreER or M: the Mdm2FM/−;CreER mice. Casp3: Caspase 3. Scale bar, 100 μm. Arrowheads indicate hippocampus. Arrows indicate inner nuclear layer of retina. * p<0.05. The number of animals in each sample group is indicated on the top of each respective bar in B.