Figure S1.

Robustness of Spores Made by the YhjH-Overexpression Strain and the bldD Mutant and Overexpressing Catalytically Inactive Versions of CdgB and YhjH Has No Effect on Development, Related to Figure 1

(A) Spore heat resistance was determined as described previously (Molle et al., 2000). The bldD mutant spore defect is mild; for comparison, under the same conditions (50°C) whiD mutant spore viability drops by a factor of 10⁸ in 30 min (Molle et al., 2000).

(B) Scanning electron micrographs showing that overexpression of catalytically inactive versions of YhjH or CdgB has no effect on S. venezuelae development. A tag-less variant of cdgB carrying a G472A mutation in the GGDEF motif was PCR-amplified from pIJ10361 (Tran et al., 2011). CdgB-AGDEF and the inactive variant of YhjH with the E48A mutation in the EAL-motif (Pesavento et al., 2008) were used for overexpression from the ermEp^∗ promoter. Cells were grown on MYM for 4 days at 30°C.