Table 2. Mutagenesis to disrupt the antigenicity of epitope-M201.
| Constructs | Sequence | IFAa | Virus recovery |
|---|---|---|---|
| Wil d type | 161AVKQGVVNLVKYAK174 | +++ | +++ |
| Mut-1 | .AAA.......... | - | - |
| Mut-2 | ....AAA....... | - | - |
| Mut-3 | .......AAA.... | - | - |
| Mut-4 | ..........AAAA | +++ | NDb |
| Mut-5 | ..AA.......... | - | - |
| Mut-6 | .......A..A..A | - | - |
IFA: indirect immunofluorescence assay with the MAb-201.
ND, not done.