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. 2014 Sep 2;9(9):e105683. doi: 10.1371/journal.pone.0105683

Figure 2. Stabilization of Peptide Ligands by nGLP-1R.

Figure 2

A ‘perturbation map’ showing changes to deuterium exchange for peptide ligands in the presence and absence of nGLP-1R. Deuterium exchange was measured in the presence of DDM/CHS micelles as indicated in the Experimental section. Resolved peptides are indicated with rectangular boxes below the sequence of exendin-4 and exendin-4[9-39]. The whiskers on the left side of each bar indicate amino acids that are included in the detected peptides, but whose amides do not report on deuterium in-exchange. As a result of digestion the first amino acid no longer contains an exchangeable amide and the back exchange rates are higher for the second amino acid. The average % change in deuterium in the presence of nGLP-1R is included inside the boxes with standard error in parenthesis. Boxes are colored according to the key where significant changes are determined by t-test. The peptide enclosed in the red box was chosen for ETD fragmentation.