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Culture hESCs on hAF-AFSCs feeder layer with exogenous b-FGF supplementation. A. Morphology of hESCs cultured on hAF-AFSCs feeder layer. B-G. Immunofluorescence staining of hESCs cultured on hAF-AFSCs feeder layer with Oct4, Nanog, SSES-3/4, Tra-1-60 and Tra-1-81.
