Perfusion images of skin flaps at 3 and 5 days postoperatively. The color scale of all images was defined by setting the lowest perfusion value to 0 and the highest perfusion value to 300. (A) IR3d, (B) HBO-PC3d, (C) IR5d, (D) HBO-PC5d and (E) SH groups. (F) Perfusion in the IR and HBO-PC groups was significantly lower than that in the SH group (**P<0.01). Compared with the same day IR groups, perfusion was significantly increased in the HBO-PC groups (##P<0.01). IR, ischemia reperfusion; HBO-PC, hyperbaric oxygen preconditioning; SH, sham-operated.