Figure 3.

A and B. Abnormal expression of SEMA3A promotes cell migration in SGC-7901 cells as demonstrated by transwell assays (**P < 0.001). Representative photos of stained cells are shown with the original magnification of 100×. C. Abnormal expression of SEMA3A inhibits cell proliferation in SGC-7901 cells (P = 0.027). Cell numbers were evaluated with the MTT assay using absorbance readings at 490 nm. The values shown are the mean of three determinations. D and E. SGC-7901 cells were transfected with pcDNA3.1 (+)-SEMA3A or empty vector for 12, 24, 48 and 72 h, respectively. (*P < 0.05 compared to empty vector).