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Detection of VP1 protein of EV71 by immunohistochemistry staining method. In cerebella, 2/3 cerebellar granule cells were strongly positive and moderate numbers of Purkinje cells displayed an intracytoplasmic and intranuclear staining (A); Neuron was negative for EV71 antigen in the brain (B); Neuron were partly positive in the brainstem (C).
