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. 2014 Sep 2;9(9):e106232. doi: 10.1371/journal.pone.0106232

Figure 1. Optimization of PURE system as measured by active Fluc produced by supplementing different concentrations of EF-Tu, Ts, G; EF4; RF1, 2, 3 and RRF.

Figure 1

(a). Active Fluc produced at different EF-Ts, Tu and G concentrations. The table below shows the actual concentration increase of EF-Ts, Tu and G in the PURE system. (b). Active Fluc produced at different EF4 concentrations. (c). Active Fluc produced at different RF1, 2, 3 and RRF concentrations. The table below shows the actual concentration increase of RF1, 2, 3 and RRF in the PURE system. Fluc activities were measured in relative luminescence unit by luciferase assay and PURE system reaction without supplement was set as control. Error bars are ± standard deviations, with n = 3.