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Detailed sequences of primers used for the PCR-HRM reaction with their characteristic starter binding temperature for complementary DNA matrix sites (1:50).
| SNP name | Primer sequence (5′→3′) | Starter binding temperature |
|---|---|---|
| C−509T | F: GTGTCTGCCTCCTGACCCTCC R: GCCTCCGGAGGGTGTCAGTG |
62°C |
| C+466T | F: CATGTCCTCACCTGGTACAGC R: CCTGAACCCGTGTTGCTCTC |
64°C |
| T+869C | F: CTGTTCGCGCTCTCGGCAG R: CCAGTAGCCACAGCAGCGG |
68°C |
F, forward; R, reverse; PCR-HRM, polymerase chain reaction-high-resolution melting.
