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. 2014 Jul 14;34(4):997–1003. doi: 10.3892/ijmm.2014.1851

Figure 1.

Figure 1

Isolation and cultivation of human sweat gland epithelial cells (hSGECs). (A) Isolation of human sweat glands following collagenase digestion. (B) Typical morphology of epithelial cells growing out from a human sweat gland fragment. Morphological characteristics of primary SGECs cultured under serum-containing conditions. (C) SGECs resembled keratinized cells and (D) the SGECs (S) usually contaminated by fibroblasts (F). (E) Morphological characteristics of primary SGECs cultured under serum-free conditions. (F) Morphological characteristics of first-passage SGECs cultured in keratinocyte serum-free medium with 1% fetal bovine serum (FBS). SGECs demonstrated good growth and displayed an epithelial-like morphology with a rounded large nucleus. Scale bar: (A) 200 μm, (B–E) 100 μm, (F) 50 μm.