Figure 6.

Reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical staining revealed the expression of leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5) in human sweat gland epithelial cells (hSGECs). (A) The specific expression of LGR5 was confirmed in the SGECs by RT-PCR; a negative control was established by using H₂O as the template. (B) Immunofluorescent staining revealed the specific expression of LGR5 in human SGECs. (C) Negative control. (D) The epithelial cells in sweat glands of the skin tissue also expressed LGR5. (E) Negative control. Scale bars: (B and C) 50 μm, (D and E) 100 μm.