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Expression of GPR40 mRNA detected by RT-PCR in different tissues. Lanes 1 and 4 show the expression of GPR40 in the pancreas (lane 4 is from a 1/10 dilution of RT product from the lane 1 sample). Lane 2 is from whole nodose ganglia. Lane 3 is from a single cultured nodose neuron. Lane 5 is from whole nodose ganglia total RNA without RT reaction, as a negative control. Lane M, DNA size marker.
