Skip to main content
. 2014 May 30;307(2):L197–L204. doi: 10.1152/ajplung.00266.2013

Fig. 2.

Fig. 2.

The miR-17-5p antagomir decreased arginase II protein levels. A: miR-17-5p antagomir decreased the miR-17-5p mRNA expression in hPASMC exposed to 21% O2 or 1% O2 for 48 h (n = 3 for each group). miR-17-5p levels were analyzed by quantitative real-time PCR and normalized to RNU48 expression using the ΔΔCT method. Data are shown as means ± SE relative to respective normoxia controls at each time point. *Different from negative control in normoxia and hypoxia, P < 0.05. B: arginase II protein levels are decreased by the miR-17-5p antagomir. hPASMC were exposed to 21% O2 or 1% O2 for 48 h. Representative Western blots for arginase II and β-actin. C: arginase II protein expression levels normalized to β-actin. Data are shown as means ± SE. §Negative control exposed to 21% O2 different from negative control exposed to 1% O2, P < 0.05. **During hypoxia, hPASMC transfected with the miR-17-5p antagomir different from negative controls, P < 0.01.