Fig. 5.

Arginase II overexpression increased miR-17-5p expression. hPASMC were transfected with recombinant adenoviral vectors carrying either green fluorescent protein (AdGFP) or the human arginase II gene (AdArgII) and then exposed to 21% O₂ or 1% O₂ for 48 h (n = 3 for each group). A: representative Western blots are shown for arginase II, c-myc, p53, and β-actin in hPASMC incubated in normoxia or hypoxia for 48 h. B: relative miR-17-5p expression normalized to normoxic controls. §hPASMC transfected with AdGFP exposed to 21% O₂ different from those exposed to 1% O₂, P < 0.05. **hPASMC transfected with AdArgII different from those transfected with AdGFP exposed to 21% O₂ or 1% O₂, P < 0.01.