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. 2014 May 30;307(2):L197–L204. doi: 10.1152/ajplung.00266.2013

Fig. 6.

Fig. 6.

Arginine or ornithine did not affect hypoxic miR-17-5p or arginase II expression. hPASMC were transfected with arginase II-siRNA (Arg2-siRNA) or scramble siRNA, and different concentrations of l-arginine (3 or 10 mM) or l-ornithine (3 or 10 mM) were added to the media and then exposed to 21% O2 or 1% O2 for 48 h (n = 3 for each group). Transfected with scramble siRNA in 21% O2 (NC) or 1% O2 (HC); transfected with Arg2-siRNA in 21% O2 (NS) or 1% O2 (HS); transfected with Arg2-siRNA and l-arginine (3 or 10 mM) added to medium in 21% O2 (NS-LA3 and NS-LA10, respectively) or 1% O2 (HS-LA3 and HS-LA10, respectively); transfected with Arg2-siRNA and l-ornithine (3 or 10 mM) added to the medium in 21% O2 (NS-LO3 and NS-LO10, respectively) or 1% O2 (HS-LO3 and HS-LO10, respectively). A: relative miR-17-5p levels. B: arginase II mRNA levels. C: the relationship between changes in miR-17-5p levels and arginase II levels. D: l-arginine has little effect on the expression of miR-17∼92 family members in normoxia. hPASMC were incubated with 10 mM l-arginine in normoxia for 48 h, and levels of miR-17-5p, miR-17–3p, miR-20a, and miR-92a were determined.