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. 2014 Jan 13;74(5):912–920. doi: 10.1136/annrheumdis-2013-204343

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Differential autophagy in human B cell subsets. (A) The increase in autophagosome density seen in CD19⁺ B cells with multispectral imaging flow cytometry is also demonstrated using autophagosomotropic dye. (B) Gating strategy for naïve (CD27⁻IgD⁺), memory (CD27⁺IgD⁻), and plasmablast (CD27⁺⁺IgD⁻) B cells, and differential autophagy by subset (C). (D) Example histogram of p62 MFI in naïve B cells of systemic lupus erythematosus (red line) compared with control (blue line). (E) Differential p62 expression in B cell subsets. *p<0.05; ***p<0.001.