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. 2014 Fall;13(3):516–528. doi: 10.1187/cbe.14-04-0066

Table 3.

Threshold concepts resulting from project phases

Phase 2 Phase 3 Phases 4 and 5
Individual versus populations of molecules Individual versus populations of molecules Steady state
Energy transfer and transformation Energy transfer and transformation Biochemical pathway dynamics and regulation
Steady-state and open systems Equilibrium, including ideas related to steady state, open systems, reversibility of processes, and dynamic processes Physical basis of interactions
Reversibility of processes/reactions (equilibrium) Thermodynamics of macromolecular structure formation
Intermolecular and intramolecular interactions (how molecules interact) Intermolecular and intramolecular interactions Free energy
Spatial/scale relationships Spatial/scale relationships
Entropy/enthalpy/free energy Entropy/enthalpy/free energy
pH, pKa, and charge pH, pKa, and charge
Biochemical visualization
Moving between different structural representations of molecules New concept added in phase 3: regulation
Reading and understanding chemical formulas and structures
Interpreting graphs
Mental models and text representations
Biochemical visualization
Probabilistic thinking
Dynamic/fluctuating processes
Randomness versus directedness