. Author manuscript; available in PMC: 2014 Sep 2.

Published in final edited form as: Biometals. 2009 Sep 17;23(1):51–58. doi: 10.1007/s10534-009-9266-8

Table 1.

Primers of genes analyzed by qRT-PCR

Gene name	Primers	Sequence (5′ → 3′)	Annealing temperature	Amplicon size (bp)
ZnT1	Forward	CACGCTAGTGGCTAACACCA	60	296
ZnT1	Reverse	AGGAAAACACGGGTTCACAC	60	296
ZnT2	Forward	TGCTCGTGTACCTGGCTGTA	60	138
ZnT2	Reverse	TCCATGTCCAGACTGATGGA	60	138
ZnT3	Forward	GCAGAGTATGCACCACTGGA	60	202
ZnT3	Reverse	CAAGGGCGCAGATAGAGAAG	60	202
ZnT4	Forward	CCTTTGGATTTCATCGCCTA	60	144
ZnT4	Reverse	GTTCTCTGCACAGCCTCGTA	60	144
Zip1	Forward	AAGCCTAGTGAGCTGCTTCG	58	148
Zip1	Reverse	ATGGCCAGGATGAACTCTTG	58	148
Zip2	Forward	TTCAGAAATTCGTGGTGCAG	58	140
Zip2	Reverse	GCGACTCCAAAAGGAAGACA	58	140
Zip3	Forward	CGTCTTCCTGGCTACATGCT	58	148
Zip3	Reverse	TCCACGAACACAGTGAGGAA	58	148
Zip4	Forward	ATGAGCTGCCTCACGAACTT	58	130
Zip4	Reverse	CTGCTAGAGCCACGTAGAGG	58	130
18s	Forward	GGACCAGAGCGAAAGCATTTGC	60	115
18s	Reverse	CGCCAGTCGGCATCGTTTATG	60	115