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. 2014 Sep 2;9(9):e106247. doi: 10.1371/journal.pone.0106247

Table 2. Oligonucleotides used in the present study.

Name Sequence Restriction site
Cj17LSal GCTGTCGAC TGATAAGAAAGAATATTG SalI
Cj17RBgl TTCAGATCT CTAATGTGTTTAGCAGGC BglII
Cj872dw CTGCCCGGGATCGATGGATCCGTA TATCTCATAAACTTGCTGATG SmaI, ClaI, BamHI
Cj872up TACGGATCCATCGATCCCGGGCAG ATCTTTACCATTTTGCTC BamHI, ClaI, SmaI
Cj864LM TGAGGATCCATGG AAAGCAAGCTAATG BamHI, NcoI
Cj864LS GATCTAGA CCTATTCTTGATTTTTAG XbaI
Cj864RM GCAGAATTC TCTTTAGTAATTTCAATC EcoRI
Cj864RS TATGGATCC TTTACCTTCACTTAATG BamHI
Cj864RX CGCTCTAGA AAGCAATGAATGTAAGTAA XbaI
Cj865RS CAGGTCGACCAATTATTTAAGACATCCTA SalI
Cjj882bis ATGGTTTATAGCGCAACAGC Ø
Cjj884_1 TGCCTCAAGGTGCGCCTGAC Ø
C8J_0813_up TCTCTAACTCAATATGAA CC Ø
C8J_0814_dw2 GTATCGTCTTATCAAAGCTG Ø
Cjj880Pag GCGTCATGA AAGGTAAAGAATATGTAATTC PagI
Cjj880Xh GTGCTCGAG TTGCTTGCTAAGTTCTTTAG XhoI
Cjj880B ATCGGATCCA CCTAGATTATTCTACTTTG BamHI
Cjj883Nc GCCATGG ATAATAGTTTTATTACTCTT NcoI
Cjj883Xh GTGCTCGAG TTTCATATTACTTAATTT XhoI
Cjj881_L TTATGGAACCTTGCGAACAATG Ø
Cjj881X AGTTCTAGA AAATGTGCTATACAAGTAAG XbaI
Cjj881_RT ATAACAATCGCCAATGC Ø
astA_Sal TTAGTCGAC ATGGTTATGTCTTAAGTG SalI
astA_Xba GTTTCTAGA ACTATCAATTCTCCAGCC XbaI
astA_Xba2 AGTTCTAGA ATCATAATTCCACGATTG XbaI
astA_RT TCTCTTCCTAAGATATCG Ø
C1Afor AATTTGTTCATGGAACGTTTGCAAATGCTGCAAATGGACAAACACC AclI
C1Arev GGTGTTTGTCCATTTGCAGCATTTGCAAACGTTCCATGAACAAATT AclI
C2Afor GGGAACTTATATCACAGCTGAAGAAAATTTTGATG PvuII
C2Arev CATCAAAATTTTCTTCAGCTGTGATATAAGTTCCC PvuII
C3Afor GTATTGTAGGTGCAACTCCCATGGATAGAGCTGAATGGATAGCAAGC NcoI
C3Arev GCTTGCTATCCATTCAGCTCTATCCATGGGAGTTGCACCTACAATAC NcoI
C4Afor CGAAAGTATGGGAAATAACGCCATGCTAGCAGCAAATCC Ø
C4Arev GGATTTGCTGCTAGCATGGCGTTATTTCCCATACTTTCG Ø
C5Afor GCTTTTTAACAGGGCCTATTGCAGCTGAATTAACAGGGATTGC PvuII
C5Arev GCAATCCCTGTTAATTCAGCTGCAATAGGCCCTGTTAAAAAGC PvuII

Bold letters indicate C. jejuni nucleotide sequences; restriction recognition sites introduced for cloning purposes are underlined, complementary fragments of primers Cj872up and Cj872dw are marked with italics. Most primers were based on the C. jejuni 81116 nucleotide sequence, but for some experiments, previously designed primers based on the C. jejuni NCTC or 81–176 nucleotide sequences were used, or primers were designed to introduce point mutations. Their single pair mismatches with C. jejuni 81116 are double underlined.