Table 2. Oligonucleotides used in the present study.
Name | Sequence | Restriction site |
Cj17LSal | GCTGTCGAC TGATAAGAAAGAATATTG | SalI |
Cj17RBgl | TTCAGATCT CTAATGTGTTTAGCAGGC | BglII |
Cj872dw | CTGCCCGGGATCGATGGATCCGTA TATCTCATAAACTTGCTGATG | SmaI, ClaI, BamHI |
Cj872up | TACGGATCCATCGATCCCGGGCAG ATCTTTACCATTTTGCTC | BamHI, ClaI, SmaI |
Cj864LM | TGAGGATCCATGG AAAGCAAGCTAATG | BamHI, NcoI |
Cj864LS | GATCTAGA CCTATTCTTGATTTTTAG | XbaI |
Cj864RM | GCAGAATTC TCTTTAGTAATTTCAATC | EcoRI |
Cj864RS | TATGGATCC TTTACCTTCACTTAATG | BamHI |
Cj864RX | CGCTCTAGA AAGCAATGAATGTAAGTAA | XbaI |
Cj865RS | CAGGTCGACCAATTATTTAAGACATCCTA | SalI |
Cjj882bis | ATGGTTTATAGCGCAACAGC | Ø |
Cjj884_1 | TGCCTCAAGGTGCGCCTGAC | Ø |
C8J_0813_up | TCTCTAACTCAATATGAA CC | Ø |
C8J_0814_dw2 | GTATCGTCTTATCAAAGCTG | Ø |
Cjj880Pag | GCGTCATGA AAGGTAAAGAATATGTAATTC | PagI |
Cjj880Xh | GTGCTCGAG TTGCTTGCTAAGTTCTTTAG | XhoI |
Cjj880B | ATCGGATCCA CCTAGATTATTCTACTTTG | BamHI |
Cjj883Nc | GCCATGG ATAATAGTTTTATTACTCTT | NcoI |
Cjj883Xh | GTGCTCGAG TTTCATATTACTTAATTT | XhoI |
Cjj881_L | TTATGGAACCTTGCGAACAATG | Ø |
Cjj881X | AGTTCTAGA AAATGTGCTATACAAGTAAG | XbaI |
Cjj881_RT | ATAACAATCGCCAATGC | Ø |
astA_Sal | TTAGTCGAC ATGGTTATGTCTTAAGTG | SalI |
astA_Xba | GTTTCTAGA ACTATCAATTCTCCAGCC | XbaI |
astA_Xba2 | AGTTCTAGA ATCATAATTCCACGATTG | XbaI |
astA_RT | TCTCTTCCTAAGATATCG | Ø |
C1Afor | AATTTGTTCATGGAACGTTTGCAAATGCTGCAAATGGACAAACACC | AclI |
C1Arev | GGTGTTTGTCCATTTGCAGCATTTGCAAACGTTCCATGAACAAATT | AclI |
C2Afor | GGGAACTTATATCACAGCTGAAGAAAATTTTGATG | PvuII |
C2Arev | CATCAAAATTTTCTTCAGCTGTGATATAAGTTCCC | PvuII |
C3Afor | GTATTGTAGGTGCAACTCCCATGGATAGAGCTGAATGGATAGCAAGC | NcoI |
C3Arev | GCTTGCTATCCATTCAGCTCTATCCATGGGAGTTGCACCTACAATAC | NcoI |
C4Afor | CGAAAGTATGGGAAATAACGCCATGCTAGCAGCAAATCC | Ø |
C4Arev | GGATTTGCTGCTAGCATGGCGTTATTTCCCATACTTTCG | Ø |
C5Afor | GCTTTTTAACAGGGCCTATTGCAGCTGAATTAACAGGGATTGC | PvuII |
C5Arev | GCAATCCCTGTTAATTCAGCTGCAATAGGCCCTGTTAAAAAGC | PvuII |
Bold letters indicate C. jejuni nucleotide sequences; restriction recognition sites introduced for cloning purposes are underlined, complementary fragments of primers Cj872up and Cj872dw are marked with italics. Most primers were based on the C. jejuni 81116 nucleotide sequence, but for some experiments, previously designed primers based on the C. jejuni NCTC or 81–176 nucleotide sequences were used, or primers were designed to introduce point mutations. Their single pair mismatches with C. jejuni 81116 are double underlined.