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. 2014 Sep 2;9(9):e106309. doi: 10.1371/journal.pone.0106309

Figure 3. wee-1.1 expression in the early embryo is Wnt/MAPK-dependent.

Figure 3

Top three rows show three time points of the same embryos from early 2E to late 2E stage. DIC images (left-hand column) and fluorescence micrographs (right-hand column) of embryos expressing the wee-1.1 reporter. The bottom row shows the expression of the same transgene in 2E-staged lit-1(t1512), left, and mom-2(or42), right, mutant embryos. Closed arrowheads indicate the 2E nuclei. Open arrowheads indicate the 2MS nuclei. Arrows point to AB nuclei expressing the reporter GFP. Note the reduced (but not absent) expression of the wee-1.1 reporter in the 2E nuclei in the lit-1 and mom-2 mutants. Bar: 10 µm.