Table 1. Coexpression of gut reporter GFP and intestinal granules in embryos defective in P2-EMS signaling.
| mutation | end-1 reporter | sdz-23 reporter | combined | |||||||||
| GFP+ | GFP- | GFP+ | GFP- | GFP+ | GFP- | |||||||
| GG+ | GG- | GG+ | GG- | GG+ | GG- | GG+ | GG- | GG+ | GG- | GG+ | GG- | |
| Wildtype | 60 | 0 | 0 | 0 | 44 | 0 | 0 | 0 | 104 | 0 | 0 | 0 |
| mom-1(or10) | 1 | 5 | 0 | 3 | 8 | 6 | 0 | 2 | 9 | 11 | 0 | 5 |
| mom-1(se2) | 6 | 1 | 1 | 5 | - | - | - | - | 6 | 1 | 1 | 5 |
| mom-3(or78) | 11 | 5 | 2 | 7 | 10 | 11 | 1 | 19 | 21 | 16 | 3 | 26 |
| mom-5(RNAi) | 20 | 2 | 0 | 0 | - | - | - | - | 20 | 2 | 0 | 0 |
| mom-4(ne19) | 10 | 7 | 0 | 0 | 2 | 2 | 0 | 0 | 12 | 9 | 0 | 0 |
| mom-4(or39) | 14 | 7 | 0 | 0 | - | - | - | - | 14 | 7 | 0 | 0 |
| mom-4(or11) | 10 | 0 | 0 | 0 | - | - | - | - | 10 | 0 | 0 | 0 |
Number of embryos (N) expressing one of two E lineage-specific reporters: Pend-1::gfp::h2b (end-1 reporter) or Psdz-23::gfp::h2b (sdz-23 reporter). The end-1 reporter was tested in wildtype (WT) embryos (N = 60), embryos mutant in the Wnt pathway: mom-1(or10), N = 9; mom-1(se2), N = 13; mom-3(or78), N = 25; mom-5(RNAi), N = 22; or embryos mutant in the MAPK pathway: mom-4(ne19), N = 17; mom-4(or39), N = 21; mom-4(or11), N = 10. The sdz-23 reporter was tested in WT, N = 44; mom-1(or10), N = 16; mom-3(or78), N = 41; and mom-4(ne19), N = 4.
Reporter expression results (GFP+/−) are correlated with gut granule development (GG+/−) for each individual embryo, generating 4 classes: GFP+/GG+, GFP−/GG+, GFP+/GG−, and GFP−/GG−.