Genomic DNA and corresponding clones of the SH60 fragment illustrating that Cx. pipiens f. molestus contains a single SH60 variant, whereas Cx. p. pipiens specimens contain multiple variants that are additive to form the complex banding patterns seen in Fig. 1. (A) NYC Cx. p. pipiens and Cx. pipiens f. molestus. Lane 1, Cx. pipiens f. molestus genomic DNA; lanes 2–5, corresponding clones from the same specimen; lane 6, Cx. pipiens f. molestus genomic DNA; lanes 7–8, SH60 clones; lane 9, Cx. p. pipiens genomic DNA; and lanes 10–12, SH60 clones. (B) NYC and Syracuse Cx. p. pipiens. Lane 1, Syracuse Cx. p. pipiens genomic DNA; lanes 2–6, SH60 clones; lane 7, NYC Cx. p. pipiens genomic DNA; and lane 8, one corresponding clone.