Table 2. Identification of homologs of meiosis-specific genes in the sequenced genome of Verticillium dahliae isolate VdLs.17.
Genea | Aspergillus fumigatusAccession | Verticillium dahliaAccession | Total alignmentScore | Expect Value | % Identity | Protein Domains |
SPO11 | XP_747937.2 | EGY19025 | 165 | 2e-50 | 32 | Conserved |
REC8 | XP_747786 | EGY21140 | 143 | 2e-39 | 27 | Conserved |
RAD21 | XP_749714.1 | EGY18368 | 447 | e-152 | 47 | Conserved |
RAD51 | XP_752407 | EGY18462 | 653 | 0 | 89 | Conserved |
MSH4 | XP_749950 | EGY21332 | 627 | 3e-153 | 42 | Partially Conserved |
MSH2 | XP_754634.1 | EGY20237 | 1416 | 0 | 73 | Conserved |
MSH5 | XP_752482.2 | EGY18511 | 438 | e-122 | 41 | Partially Conserved |
MSH6 | XP_751995.1 | EGY17510 | 1392 | 0 | 61 | Conserved |
HOP1 | XP_751182.1 | No homolog found | - | - | - | - |
HOP2 | EWH18245b | No homolog found | - | - | - | - |
MND1 | XP_751862 | No homolog found | - | - | - | - |
DMC1 | XP_746712.1 | No homolog foundc | - | - | - | - |
Protein sequences of 11 meiosis-specific genes in Aspergillus fumigatus [14] and one in Saccharomyces cerevisiae (HOP2) were used to search the V. dahliae genome using blastp. Accession numbers are from GenBank.
Genes in Saccharomyces cerevisiae previously shown to function in meiosis [14].
HOP2 sequence from S. cerevisiae was used for searching the VdLs.17 genome.
Although homologs are present, protein domain analyses suggests that the best hit is the related protein encoded by RAD51, not DMC1.