Figure 3. Reporter activity over temperature range. To elucidate the temperature-dependent RNAT activity of the avashort and the avalong-5′UTRs, reporter activity of both RNAT-bgaB fusions as well as the control hsp17-bgaB were analyzed in E. coli over a temperature range from 30–42 °C in 2 degree steps. The presented results are mean values of a double measurement; mean standard deviation is indicated by error bars. For normalization, the respective 30 °C value was set to 1.