Figure 5. Identification and verification of tRNA and sRNA precursors binding to Hfq. Deep sequencing and Northern blot analyses of Spf (A and B) and the metZWV precursor (C and D) as described in Figure 3A and 3B. Violet and red bars indicate the positions of oligonucleotide probes used in the corresponding Northern blots. 6S RNA was used as a loading control. The black bar indicates the position of the Hfq aptamer reported by Lorenz et al.⁹