Fig. 4.
Protein and gene analysis for strains expressing the fusion complexes. (A) SDS–PAGE profiles of the complexes purified from cells grown under semiaerobic conditions. (B) Restriction analysis of plasmid DNA isolated from cells grown under photoheterotrophic conditions. (C) SDS–PAGE profiles of the complexes purified from cells grown under photoheterotrophic conditions. M – mass marker (kDa or kb), cyt. bSb – hybrid fusion of cytochromes b in BS–B complex, cyt. b – monomeric cytochrome b, remaining lanes descriptions correspond to the names of the mutants shown in Figs. 2 and 3.