Fig. 2.

Stimulation of adenosine signaling with NECA has no effect on TGFβ-induced expression of αSMA in cardiac Sca1⁺CD31⁻ cells in vitro. Representative cytofluorographic dot plots (a) of intracellular αSMA staining in cardiac Sca1⁺CD31⁻ cells cultured in the absence (control) or presence (TGFβ) of 1 ng/ml TGFβ1 and in the absence (−NECA) or presence (+NECA) of 10 μM NECA for 24 h. The percentages of αSMA-positive cells (b) and their mean fluorescence intensity (ΔMFI) (c) are presented as means ± SEM of three experiments. Asterisks indicate statistical differences (**p < 0.01, ***p < 0.001) and ns indicates non-significant differences analyzed by one-way ANOVA with Bonferroni post-hoc tests