Fig. 3.

IA2- and/or ATP-γ-S-induced release of IL-8 after P2Y₂ knockdown. IL-8 measured in supernatant after 5 h IA2 (10⁸ CFU/mL) and/or ATP-γ-S (20 μM) or IL-1β (10 ng/mL) stimulation of A498 cells. Cells had, prior to stimulation, been transfected with either lipofectamine alone or in combination with P2Y₂ specific siRNA. Data was normalised to obtain the actual effect of P2Y₂ knockdown and are expressed as mean percentage of control ± SEM with control shown as the horizontal line at 100 %. Asterisk represents significance between groups indicated by brackets (n = 3)