Fig. 2.

IRF8 regulates the expression of microglial motility-related genes. a Western immunoblot of phosphorylated Akt (pAkt) and total Akt in whole cell lysates of Irf8 ^+/+or Irf8 ^−/−microglial cells 15 min after ATP treatment. b Real-time PCR analysis of purinergic receptors, P2ry12 and P2rx4, and adenosine A₃ receptor, Adora3, in Irf8 ^+/+, Irf8 ^+/−, and Irf8 ^−/−microglial cells (n = 5–9). c Western immunoblot of P2X4R in whole cell lysates of Irf8 ^+/+and Irf8 ^−/−microglial cells. d Real-time PCR analysis of the ectonucleoside triphosphate diphosphohydrolase (Entpd1) and ecto-5′-nucleotidase enzyme (Nt5e) in Irf8 ^+/+, Irf8 ^+/−, and Irf8 ^−/−microglial cells (n = 5–9). *P < 0.05; **P < 0.01; ***P < 0.001 vs. Irf8 ^+/+microglial cells. Values are the mean ± SEM