Fig. 4.
Western blot analysis of the wild-type hA2BR and R103A, I107A, and L213A mutations from top to bottom panel as expressed in different MMY strains. a Gel 1: Lane 1 MMY12 carrying pDT-PGK without receptor; Lanes 2–6 MMY12, MMY14, MMY19, MMY20, and MMY21 carrying pDT-PGK_A2B wild-type or mutant receptor. Gel 2: Lane7–10 MMY23, MMY24, MMY28, and MMY12 carrying pDT-PGK_A2B mutant receptor; Lane 11 MMY12 carrying pDT-PGK without receptor. The A2B receptor specific bands are 29 and 50 kDa, which are absent in MMY12 carrying pDT-PGK without receptor; nonspecific band at approximately 45 kDa was used as loading control, which also appeared in MMY12 carrying pDT-PGK without receptor. b Bar graphs were calculated from a densitometric analysis of the blots. The ratio was determined between the density of the specific bands and that of the nonspecific band that is always present on the blots. MMY12 carrying wild-type or mutant receptor was set as 100 % and MMY12 carrying the empty vector pDT-PGK without receptor was set as 0 %