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Radioligand-binding experiments of wild-type and mutant hA2B receptors expressed in MMY24(Gαi3) using 1.5 nM [3H]PSB-603. Specific binding of wild-type receptor was set at 100 %. IC50 values were obtained from competition binding curves of five independent experiments, performed in duplicate
| Mutant | % specific binding | IC50 (μM) |
|---|---|---|
| Wild type | 100 | 1.85 ± 0.87 |
| R103A | 0 | nd |
| I107A | 12 | nd |
| L213A | 94 | 1.90 ± 1.64 |
| S235A | 20 | nd |
| L236A | 158 | 1.88 ± 0.6 |
nd not determined
